In the Fully Yoked 1.4 update to Grounded, the developers added something called Ant Queens. These aren’t bosses, but a choice given to players who have delved deep into the map’s anthills.
Grounded ULTIMATE Ant Queen Guide! All recipes and locations!
There are now three Ant Queens, one in each colony. You have a choice for each of the Queens: you can either befriend them or betray them. There is also a secret third option, which is to infect them. The Red Ant Queen, the Fire Ant Queen, and the Black Ant Queen all have hidden, unique recipes for getting infected. All three Ant Queens are available in the base game, so you don't need to start New Game Plus to interact with them. However, some of their options are only available in New Game Plus.
This is the easiest of the Ant Queens to interact with. She resides deep within the Red Ant Hill, which is located in the lower garden. She can be befriended, killed, or infected, and each choice has its own consequences.
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